Biofilm Formation by Streptococcus Serotypes on Dental Plaques
نویسنده
چکیده
A biofilm is an aggregate of microorganisms in which cells adhere to each other on a surface. The biofilm forming capacity of five isolate of streptococcus species were tested by microtitre plate assay. The assay was optimized in LB broth for 48h incubation, fixing of cells by heat and staining using solubilizing solution. The optimized microtitre plate assay can be effectively used for the assessment of biofilm ability of streptococcus strains. The highlight of study was the testing of large number of streptococcus strains belonging to several serotypes. Majority of streptococcus strains are shown to form biofilms on dental plaques.
منابع مشابه
Effect of Bacteriocin Produced by Lactobacillus casei with Probiotic Potential Isolated from Milk on the Ability of Biofilm Production by Streptococcus salivarius Obtained from Dental Plaque
Background and purpose: Dental plaque is a biofilm that is formed on the surface of the tooth. Carious lesions are caused by inappropriate ecological changes in microbial flora of the plaque biofilm. In this study, the effect of Lactobacillus casei bacteriocin, isolated from dairy products, was investigated on Streptococcus salivarius biofilm formation. Materials and methods: In this experimen...
متن کاملتأثیر عصاره های گال مازو بلوط بر رشد و تشکیل بیوفیلم استرپتوکوکوس موتانس
Background and purpose: Dental caries is a pathological infectious disease. It begins with the formation of dental plaques which is a structurally and functionally organized biofilm. Streptococcus mutans is the most important bacterium in the formation of dental plaque and dental caries. This study aimed at evaluating the antibacterial and antibiofilm activity of Quercus infectoria galls agains...
متن کاملQuorum quenching for the management of dental plaque microbes
In the present study, variation among different bacterial strains was observed in the plaques of tobacco chewers (TC) as compared to those from normal persons (non tobacco chewers) (NTC). Bacterial strain J1 (dominant in the plaques of NTC) did not initiate the process of adhesion (biofilm formation) until it appeared to have reached a required population density necessary for the production of...
متن کاملEffect of Isolated Specific Lytic Phage against Growth and Biofilm Inhibition of Streptococcus mutans and Streptococcus sanguinis Isolated from Decayed Dental Plaque
Background and purpose: Despite advances in oral health and dental industry, tooth decay remains one of the most common oral diseases. One of the new methods to combat dental plaque, which is the main cause of caries, is using specific lytic bacteriophage. This study aimed to investigate the effect of isolated specific lytic phage against growth and biofilm inhibition of Streptococcus mutans an...
متن کاملEvaluation of inhibitory effects of Chlorella vulgaris extract on growth, proliferation and biofilm formation by Streptococcus mutans and evaluation of its toxicity
Background & Objectives: Dental caries is the most important disease caused by some bacteria specially Streptococcus Mutans from Viridans family. The aim of this study is to evaluate the inhibitory effect of Chlorella vulgaris extract on growth, proliferation, and biofilm formation of Streptococcus mutans. Materials & Methods: Microalgae Chlorella vulgaris was extracted via maceration using ch...
متن کامل